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Human Coxsackievirus B4
Human Coxsackievirus B4
規(guī)格:
貨期:
編號:B162872
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Human Coxsackievirus B4
商品貨號 B162872
Classification Picornaviridae, Enterovirus, Enterovirus B
Deposited As Coxsackievirus B 4
Agent Human Coxsackievirus B4
Strain J.V.B. (Benschoten)
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
Stool from 10-year-old child, New York, 1951
Product Format frozen 1.0 mL
Storage Conditions -70°C or colder
Images ATCC VR-184 images
Effect on Host
CPE, cell rounding and sloughing
Recommended Host
LLC-MK2 Derivative (ATCC® CCL-7.1™)
Growth Conditions Temperature: 37°C
Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-7 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Name of Depositor H Plager
Source Stool from 10-year-old child, New York, 1951
Year of Origin 1951
References

Dalldorf G. The Coxsackie viruses. Bull NY Acad.. Med 26: 329-335, 1950. PubMed: 15411582

Sickles GM, et al. Isolation and type determination of Coxackie virus, group B, in tissue culture. Proc. Soc. Exp. Biol. Med. 88: 22-24, 1955. PubMed: 14357332

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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