| 產(chǎn)品名稱 | 15P-1 |
|---|---|
| 商品貨號 | B163588 |
| Organism | Mus musculus, transgenic, mouse, transgenic |
| Tissue | testis |
| Cell Type | Sertoli cell |
| Product Format | frozen |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age | 6 months |
| Gender | male |
| Storage Conditions | liquid nitrogen vapor phase |
| Derivation | The 15P-1 cell line was established from testicular cells of transgenic mice that express the large T protein of polyoma virus (PyLT) in the seminiferous epithelium.Ref   Paquis-Flucklinger V, et al. Expression in transgenic mice of the large T antigen of polyomavirus induces Sertoli cell tumours and allows the establishment of differentiated cell lines. Oncogene 8: 2087-2094, 1993. PubMed: 8393161 |
| Comments | The 15P-1 cell line was established from testicular cells of transgenic mice that express the large T protein of polyoma virus (PyLT) in the seminiferous epithelium. Ref This line exhibits characteristics of Sertoli cells, including transcription of the Wilms' tumor (WT1) and Steel genes. Ref 15P-1 cultures express phagocytic activity evidenced by the internalization of latex beads and the disposal of dead cells. Interaction with germ cells enhances the phagocytic activity of 15P-1 cells. Ref The cells support the meiotic and postmeiotic differentiation in cocultures of diploid premeiotic germ cells into haploid spermatids expressing the protamine (Prm-1) gene. Ref When cocultured with 15P-1 cells, testicular cells explanted from immature 9-day-old animals, before the onset of the first meiosis, generate tetrads of haploid cells with the morphology of round spermatids and initiate protamine transcription. Ref The 15P-1 cell line releases protease-sensitive material with broad-spectrum antibacterial activity. The activity of 15P-1 culture medium is increased 10-fold to 50-fold in the presence of fractions enriched in round spermatids and of nerve growth factor. Ref The KL protein, a ligand of the Kit receptor, is expressed in Sertoli cells and in 15P-1 cells. Ref |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:3 to 1:4 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation | Culture medium 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions | Temperature: 32°C |
| Name of Depositor | F Cuzin |
| Deposited As | mouse, transgenic |
| References | Paquis-Flucklinger V, et al. Expression in transgenic mice of the large T antigen of polyomavirus induces Sertoli cell tumours and allows the establishment of differentiated cell lines. Oncogene 8: 2087-2094, 1993. PubMed: 8393161 Rassoulzadegan M, et al. Transmeiotic differentiation of male germ cells in culture. Cell 75: 997-1006, 1993. PubMed: 7504588 Grandjean V, et al. Stage-specific signals in germ line differentiation: control of Sertoli cell phagocytic activity by spermatogenic cells. Dev. Biol. 184: 165-174, 1997. PubMed: 9142992 Grandjean V, et al. Antimicrobial protection of the mouse testis: synthesis of defensins of the cryptdin family. Biol. Reprod. 57: 1115-1122, 1997. PubMed: 9369178 Vincent S, et al. Stage-specific expression of the Kit receptor and its ligand (KL) during male gametogenesis in the mouse: a Kit-KL interaction critical for meiosis. Development 125: 4585-4593, 1998. PubMed: 9778516 Vidal F, et al. Gene trap analysis of germ cell signaling to Sertoli cells: NGF-TrkA mediated induction of Fra1 and Fos by post-meiotic germ cells. J. Cell Sci. 114: 435-443, 2001. PubMed: 11148144 |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
