| 產(chǎn)品名稱 |
1A2 |
| 商品貨號(hào) |
B163612 |
| Organism |
Homo sapiens, human |
| Cell Type |
lymphoblast B lymphocyte; Epstein-Barr virus (EBV) transforme |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
2 CELLS CONTAIN HERPESVIRUS
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Lymphoma |
| Applications |
This line was derived from the GM1500 lymphoma line. The cells are resistant to 6-thioguanine. The cells can be used as a source of infectious EBV, and can be used as driving the cell in"cell driven" EBV transformation of human B cells. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
This line was derived from the GM1500 lymphoma line. |
| Genes Expressed |
Epstein-Barr virus (EBV) |
| Cellular Products |
Epstein-Barr virus (EBV) |
| Comments |
This line was derived from the GM1500 lymphoma line. The cells are resistant to 6-thioguanine. The cells can be used as a source of infectious EBV, and can be used as driving the cell in"cell driven" EBV transformation of human B cells. |
| Complete Growth Medium |
RPMI 1640 medium with 1 mM sodium pyruvate and 2 mM L-glutamine, 85%; fetal bovine serum, 15%
|
| Subculturing |
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml and maintain between 2 X 10(5) and 1 X 10(6) cells/ml. Interval: every 3 days Medium Renewal: Every 3 days |
| Cryopreservation |
Freeze medium: culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Population Doubling Time |
24 hrs (when cells are in logarithmic growth) |
| Name of Depositor |
Genetic Systems Corp. |
| Deposited As |
Homo sapiens |
| U.S. Patent Number |
|
| References |
Lostrom ME. Cell-driven viral transfer in eukaryotes. US Patent 4,464,465 dated Aug 7 1984
Croce CM, et al. Chromosomal location of the genes for human immunoglobulin heavy chains. Proc. Natl. Acad. Sci. USA 76: 3416-3419, 1979. PubMed: 114999
|
