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The line was established from normal abdominal skin.

The line was established from normal abdominal skin.

female

Black

42 years

The line was established from normal abdominal skin.

Cells senesce after approximately 60 population doublings.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended

Medium Renewal: Every 2 to 3 days

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

culture medium 95%; DMSO, 5%

Temperature: 37.0°C