| 產(chǎn)品名稱(chēng) |
CH26-1352 |
| 商品貨號(hào) |
B164243 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with FO myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against double stranded DNA |
| Cellular Products |
immunoglobulin; monoclonal antibody; against double stranded DNA |
| Tumorigenic |
Yes |
| Effects |
Yes, forms ascites in pristane primed BALB/c mice |
| Comments |
Animals were immunized with synthetic polynucleotide homopolymers [poly(dA-dT)-poly(dA-dT) and poly(dI-dC)-poly(dI-dC)] then boosted with foreign native DNA (EcoR1 digested pBR322 DNA). Spleen cells were fused with FO myeloma cells. |
| Complete Growth Medium |
Culture Medium: Iscove's modified Dulbecco's medium, 90%; fetal bovine serum,
10%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
Culture medium, 90%; DMSO, 10% |
| Isotype |
IgM |
| Name of Depositor |
Cetus Corp. |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Huang CM, Cohen SN. Monoclonal antibody having specificity for the double-stranded conformation of native DNA and diagnostic methods using same. US Patent 4,623,627 dated Nov 18 1986
|
