| 產(chǎn)品名稱 |
HIG-82 |
| 商品貨號(hào) |
B164609 |
| Organism |
Oryctolagus cuniculus, rabbit |
| Tissue |
synovium |
| Cell Type |
synoviocyte |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age |
6 months |
| Gender |
female |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
at passage 86; modal number = 44; range = 42 to 45; five marker chromosomes were present [ PubMed: 2846503]. |
| Derivation |
This cell line was derived from the intrarticular soft tissue from the knee joint of a young female rabbit. |
| Genes Expressed |
prostaglandin E2 (PGE2); collagenase; gelatinase; caseinase (stromelysin); all are produced after activation with phorbol myristic acid (PMA) or interleukin-1 (IL-1, interleukin 1) |
| Comments |
This cell line has retained many of the features of normal rabbit synoviocytes including production of cytokines that activate primary cultures of normal chondrocytes.
These cells can be activated with PMA or IL-1, and can phagocytose latex beads. |
| Complete Growth Medium |
Ham's F12 medium, 90%; fetal bovine serum, 10%
|
| Subculturing |
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
- Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
- Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Population Doubling Time |
24 hrs |
| Name of Depositor |
HI Georgescu, CH Evans |
| Deposited As |
Oryctolagus cuniculus |
| Passage History |
Recieved at ATCC in 1988 at passage 202. |
| References |
Sung K, et al. Characterization of chondrocyte activation in response to cytokines synthesised by a synovial cell line. Biochim. Biophys. Acta 971: 148-156, 1988. PubMed: 2844284
Georgescu HI, et al. HIG-82: an established cell line from rabbit periarticular soft tissue, which retains the "activatable" phenotype. In Vitro Cell. Dev. Biol. 24: 1015-1022, 1988. PubMed: 2846503
Development and diseases of cartilage and bone matrix. New York: Liss; 1987.
Watanabe S, et al. Chondrocyte activation in response to factor(s) produced by a continuous line of lapine synovial fibroblasts. Exp. Cell Res. 167: 218-226, 1986. PubMed: 3019747
|
