| 產(chǎn)品名稱(chēng) |
IgG-6A6 |
| 商品貨號(hào) |
B164844 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
Animals were immunized with a peptide corresponding to the 10 carboxy terminal amino acids (SVVSTDDDLA) of the rat VLDL receptor coupled to keyhole limpet hemocyanin. It can be used in immunoblotting assays to detect VLDL receptors. |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against very low density lipoprotein (VLDL) receptor |
| Cellular Products |
immunoglobulin; monoclonal antibody; against very low density lipoprotein (VLDL) receptor |
| Tumorigenic |
Yes |
| Effects |
Yes, in BALB/c mice |
| Comments |
Animals were immunized with a peptide corresponding to the 10 carboxy terminal amino acids (SVVSTDDDLA) of the rat VLDL receptor coupled to keyhole limpet hemocyanin. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody recognizes the cytoplasmic domain of the VLDL receptor from various tissues and species. It can be used in immunoblotting assays to detect VLDL receptors. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Isotype |
IgG1 |
| Name of Depositor |
JL Goldstein, YK Ho |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Jokinen EV, et al. Regulation of the very low density lipoprotein receptor by thyroid hormone in rat skeletal muscle. J. Biol. Chem. 269: 26411-26418, 1994. PubMed: 7929362
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