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IM7.8.1
IM7.8.1
規(guī)格:
貨期:
編號(hào):B164857
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 IM7.8.1
商品貨號(hào) B164857
Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
The antibody may be useful for detecting subclasses of intrathymic progenitor cells and in detecting memory T cells in the periphery.
Derivation
Spleen cells were fused with S194/5.XXO.BU.1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against a non-polymorphic determinant on mouse pgp-1 glycoprotein
Cellular Products
immunoglobulin; monoclonal antibody; against a non-polymorphic determinant on mouse pgp-1 glycoprotein
Comments
Animals were immunized with dexamethasone induced cells of the M1 mouse myeloid cell line.
Spleen cells were fused with S194/5.XXO.BU.1 myeloma cells.
The antibody may be useful for detecting subclasses of intrathymic progenitor cells and in detecting memory T cells in the periphery.
Complete Growth Medium Dulbecco's modified Eagle's medium, 90%; heat-inactivated horse serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG2b
Name of Depositor R Hyman
Deposited As rat (B cell); mouse (myeloma)
References

Budd RC, et al. Distinction of virgin and memory T lymphocytes. Stable acquisition of the Pgp-1 glycoprotein concomitant with antigenic stimulation. J. Immunol. 138: 3120-3129, 1987. PubMed: 3106474

Trowbridge IS, et al. Biochemical characterization and cellular distribution of a polymorphic, murine cell-surface glycoprotein expressed on lymphoid tissues. Immunogenetics 15: 299-312, 1982. PubMed: 7068174

Wilson ME, et al. Local suppression of IFN-gamma in hepatic granulomas correlates with tissue-specific replication of Leishmania chagasi. J. Immunol. 156: 2231-2239, 1996. PubMed: 8690913

Lesley J, et al. Qualitative and quantitative heterogeneity in Pgp-1 expression among murine thymocytes. Cell. Immunol. 112: 40-54, 1988. PubMed: 2894254

Lesley J, et al. Kinetics of thymus repopulation by intrathymic progenitors after intravenous injection: Evidence for successive repopulation by an IL-2R+, Pgp-1- and by an IL-2R-, Pgp-1+ progenitor. Cell. Immunol. 117: 378-388, 1988. PubMed: 3058318

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