| 產(chǎn)品名稱 |
JH4 clone 1 |
| 商品貨號(hào) |
B164892 |
| Organism |
Cavia porcellus, guinea pig |
| Tissue |
lung |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Gender |
female |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
normal female; diploid; stable |
| Clinical Data |
female |
| Complete Growth Medium |
Ham's F12K medium, 90%; fetal bovine serum, 10%
|
| Subculturing |
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
- Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
- Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
|
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Name of Depositor |
R Hay |
| Deposited As |
Cavia porcellus |
| References |
Hoying J. Selection and histochemical identification of epithelial-like cells from guinea pig lung M.Sc. thesis, Wright State Univ., 1975
|
| Cross References |
Nucleotide (GenBank) :
AF131949
Cavia porcellus tissue factor mRNA, complete cds.
|
