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KMI6
KMI6
規(guī)格:
貨期:
編號:B164925
品牌:Mingzhoubio

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產品名稱 KMI6
商品貨號 B164925
Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antigenic specificity has now been identified as the murine integrin beta 1 homolog.
Unlike the alpha 4 specific antibody PS/2 (see ATCC CRL-1911), KMI6 did not block lymphoid cell adhesion.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse integrin beta 1 subunit (CD29)
Cellular Products
immunoglobulin; monoclonal antibody; against mouse integrin beta 1 subunit (CD29)
Comments
Animals were immunized with BMS2 murine bone marrow stromal cells.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
This line was originally characterized as a murine stromal cell restricted marker.
The antigenic specificity has now been identified as the murine integrin beta 1 homolog.
Unlike the alpha 4 specific antibody PS/2 (see ATCC CRL-1911), KMI6 did not block lymphoid cell adhesion.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isotype IgG2a
Name of Depositor PW Kincade
Deposited As rat (B cell); mouse (myeloma)
References

Wu X, et al. Recognition of murine integrin beta 1 by a rat anti-stromal cell monoclonal antibody. Hybridoma 13: 409-416, 1994. PubMed: 7532155

梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479
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