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pbcr
pbcr
規(guī)格:
貨期:
編號:B237380
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pbcr
商品貨號 B237380
Designations pbcr
Species Homo sapiens, human
Depositors T Fink
Vector
Construct size (kb): 4.5
Insert
DNA: genomic
Insert lengths(kb): 1.950000047683716
Gene product: breakpoint cluster region [BCR]
Alleles: A1, A1, A2, A2
Insert Size (kb) 1.950
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.65, 1.95; HindIII--2.65, 1.95; PstI--2.65, 1.75; SalI--2.65, 1.95; EcoRI--4.5.
This probe was originally assigned to D22S11. However, it has since been shown that D22S11 is within the BCR locus.
Insert is 1.95 kb BglII/HindIII fragment containing exon 1 given in fig. 2b. The BglII site was lost by subcloning. The insert contains 2 TaqI sites; the polymorphic one is 0.5 kb from the HindIII end.
Depositor verified that this preparation detects the TaqI RFLP.
Enzyme(s) not detecting polymorphism: RsaI, PstI, EcoRI. Tested in 12 unrelated middle-Europeans.
References

Roschmann E, et al. RFLP detected with a 5'-bcr-gene-sequence [HGM8 provisional no. D22S11]. Nucleic Acids Res. 15: 1883, 1987. PubMed: 2881280

Heisterkamp N, et al. Structural organization of the bcr gene and its role in the Ph' translocation. Nature 315: 758-761, 1985. PubMed: 2989703

Groffen J, et al. Philadelphia chromosomal breakpoints are clustered within a limited region, bcr, on chromosome 22. Cell 36: 93-99, 1984. PubMed: 6319012

Thomas Fink, personal communication

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