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pUMA23
pUMA23
規(guī)格:
貨期:
編號(hào):B243063
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) pUMA23
商品貨號(hào) B243063
Designations pUMA23
Depositors UA Heinlein
Biosafety Level 1
Vector Information
Size (kb): 3.0000000000000000
Vector: pUMA23 (plasmid)
Promoters: Promoter for in vitro transcription SP6
Construction: pSP64
Marker(s):ampR
Construct size (kb): 3.0
Features: initiation codon: ATG
marker(s): ampR
promoter for in vitro transcription: SP6
replicon: pMB1
MCS: EcoRI...NotI
restriction site: NcoI
stop codons: 3
Applications
construct to generate mRNA that can be expressed in Xenopus oocytes
vector for constructing cDNA libraries
vector permitting RNA synthesis in vitro
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0; PstI--3.0; XbaI--3.0.
Cloning vector useful for in vitro transcription of cloned inserts. In vitro transcribed sequences will all contain the authentic 5'-UTR of X. laevis beta globin on the 5' end.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Dommers S, Heinlein UA. Recombinant polypeptides transplanted from pUMA vector-derived cRNAs are translocated through microsomal membranes and exported out of frog oocytes. Biochem. Biophys. Res. Commun. 204: 1346-1351, 1994. PubMed: 7980614

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梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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