| 產(chǎn)品名稱 |
McA-RH7777 |
| 商品貨號(hào) |
B165104 |
| Organism |
Rattus norvegicus, rat |
| Tissue |
liver |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
loosely adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
hepatoma; Morris hepatoma 7777 |
| Gender |
female |
| Strain |
Buffalo |
| Applications |
This cell line is a suitable transfection host.
|
| Storage Conditions |
liquid nitrogen vapor phase |
| Clinical Data |
female |
| Receptor Expression |
glucocorticoid |
| Genes Expressed |
alpha-fetoprotein (AFP, alpha fetoprotein) |
| Cellular Products |
alpha-fetoprotein (AFP, alpha fetoprotein) |
| Comments |
Addition of glucocorticoids (dexamethasone) to the medium accelerates cell proliferation and reduces alpha fetoprotein production. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Heavy monolayer sloughs off; subculture before 70% confluency.
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
- Remove culture medium with floating cells to a centrifuge tube.
- If any cells are attached, tap flask gently or if necessary add 2.0 to 3.0 mL of 0.25% Trypsin-0.53 mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed.
- Add 2.0 to 3.0 mL of complete growth medium and aspirate cells by gently pipetting.
- To remove trypsin-EDTA solution, transfer cell suspension to the centrifuge tube with the medium and cells from step #1 and spin at approximately 125 x g for 5 to 10 minutes.
- Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.
- Place culture vessels in incubators at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 weekly is recommended
Medium Renewal: Add medium every 2 to 3 days, do not discard floating cells. |
| Cryopreservation |
Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Name of Depositor |
JE Becker |
| Deposited As |
Rattus sp. |
| References |
Morris H, Meranze D. Induction and Some Characteristics of “Minimal Deviation” and Other Transplantable Rat Hepatomas. Recent Results Cancer Res. 44: 103-114, 1974.
Kulas DT, et al. The transmembrane protein-tyrosine phosphatase LAR modulates signaling by multiple receptor tyrosine kinases. J. Biol. Chem. 271: 748-754, 1996. PubMed: 8557682
Schock D, et al. An auxiliary factor containing a 240-kDa protein complex is involved in apolipoprotein B RNA editing. Proc. Natl. Acad. Sci. USA 93: 1097-1102, 1996. PubMed: 8577721
Becker JE, et al. Two new rat hepatoma cell lines for studying the unbalanced blocked ontogeny hypothesisIn: Becker JE, et al. Onco-developmental gene expression. New YorkAcademic Press, pp. 259-270, 1976
|
| Cross References |
Nucleotide (GenBank) :
X56145
Rat mRNA for oncofetal protein (ORF-1A).
|
