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MLH2
MLH2
規(guī)格:
貨期:
編號(hào):B165166
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 MLH2
商品貨號(hào) B165166
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
The antibody reacts specifically with the 28000 dalton polypeptide of the chloroplast thylakoid.
This antibody and that of MLH1 (see ATCC CRL-1766) may be useful in studies of the LHC-II gene family (Cab) and in studying the photoregulation of the Cab genes in chloroplast development.
Tested and found negative for ectromelia virus (mousepox).
Storage Conditions liquid nitrogen vapor phase
Derivation
Animals were immunized with purified LHC-II.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against the light harvesting chlorophyll a/b protein complex (LHC-II) of higher plants
Cellular Products
immunoglobulin; monoclonal antibody; against the light harvesting chlorophyll a/b protein complex (LHC-II) of higher plants
Tumorigenic Yes
Effects
Yes, in BALB/c mice
Comments
Animals were immunized with purified LHC-II.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts specifically with the 28000 dalton polypeptide of the chloroplast thylakoid.
This antibody and that of MLH1 (see ATCC CRL-1766) may be useful in studies of the LHC-II gene family (Cab) and in studying the photoregulation of the Cab genes in chloroplast development.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium:
  • 0.05 mM 2-mercaptoethanol
  • fetal bovine serum to a final concentration of 10%
Subculturing

Scrape attached cells. Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 viable cells/mL. Maintain cultures at a cell concentration between 1 x 105 and 1 x 106 cells/mL. Do not allow the cell concentration to exceed 1 x 106 cells/mL.

Medium Renewal: Two to three times weekly
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype IgG1
Name of Depositor SC Darr
Deposited As mouse (B cell); mouse (myeloma)
References

Darr SC, et al. Monoclonal antibodies to the light-harvesting chlorophyll a/b protein complex of photosystem II. J. Cell Biol. 103: 733-740, 1986. PubMed: 3528171

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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