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MLTC-1
MLTC-1
規(guī)格:
貨期:
編號:B165167
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 MLTC-1
商品貨號 B165167
Organism Mus musculus, mouse
Tissue testis
Cell Type Leydig cell
Product Format frozen
Morphology epithelial
Culture Properties adherent, Cells form islands that dome and the monolayer may not become confluent.
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Leydig cell tumor
Gender male
Strain C57BL/6
Applications This cell line is a suitable transfection host.
Karyotype polyploid; modal number = 95 RefRebois RV. Establishment of gonadotropin-responsive murine leydig tumor cell line. J. Cell Biol. 94: 70-76, 1982. PubMed: 6288740
Derivation
This line was established from the M548OP transplantable Leydig cell tumor carried in C57BL/6 mice.RefRebois RV. Establishment of gonadotropin-responsive murine leydig tumor cell line. J. Cell Biol. 94: 70-76, 1982. PubMed: 6288740
Clinical Data
male
Receptor Expression
chorionic gonadotropin; luteinizing hormone (LH)
Genes Expressed
progesterone
Cellular Products
progesterone
Tumorigenic Yes
Effects
Yes, in C57BL/6 mice
Comments
The cells retain the hormonal responsiveness of the original tumor.
Membrane adenyl cyclase activity is stimulated by treatment with human chorionic gonadotropin (hCG), luteinizing hormone, cholera toxin, sodium fluoride and guanyl-5'-ylimidodiphosphate, and produce progesterone in response to hCG. RefRebois RV. Establishment of gonadotropin-responsive murine leydig tumor cell line. J. Cell Biol. 94: 70-76, 1982. PubMed: 6288740
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 3 to 4 days
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Population Doubling Time 35 to 40 hrs
Name of Depositor RV Rebois
Deposited As Mus musculus
References

Rebois RV. Establishment of gonadotropin-responsive murine leydig tumor cell line. J. Cell Biol. 94: 70-76, 1982. PubMed: 6288740

Rebois RV. Establishment of gonadotropin-responsive murine leydig tumor cell line. J. Cell Biol. 94: 70-76, 1982. PubMed: 6288740

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