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MMQ
MMQ
規(guī)格:
貨期:
編號(hào):B165176
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) MMQ
商品貨號(hào) B165176
Organism Rattus norvegicus, rat
Tissue pituitary
Product Format frozen
Morphology epithelial
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease tumor
Age 5 days
Applications
The cells secrete prolactin and express functional dopamine receptors [U.S. Patent 5,510,260].
Storage Conditions liquid nitrogen vapor phase
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Receptor Expression
dopamine
Genes Expressed
prolactin
Cellular Products
prolactin
Tumorigenic No
Effects
No, in immunosuppressed mice
Yes, in semisolid medium
Comments
The cells secrete prolactin and express functional dopamine receptors [U.S. Patent 5,510,260].
Complete Growth Medium The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 2.5%
  • horse serum to a final concentration of 15%
    • Subculturing
    Cultures can be maintained by addition or replacement of medium. Establish new cultures at 2 x 105 viable cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL.
    Medium Renewal: Every 2 to 3 days
    Note: The line grows as clumps of cells in suspension.
    Cryopreservation

    Complete culture medium described above supplemented with 5% (v/v) DMSO. 

    Cell culture tested DMSO is available as ATCC® Catalog No. 4-X.
    Culture Conditions Temperature: 37°C
    Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
    Population Doubling Time 24 to 48 hrs
    Name of Depositor University of Virginia Patent Foundation
    U.S. Patent Number
    References

    MacLeod RM, et al. Prolactin secreting cell line and method of obtaining the same. US Patent 5,510,260 dated Apr 23 1996

    The MMQ cell line was established from the 7315a rat pituitary tumor.

    Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

    Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

    Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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