| 產(chǎn)品名稱(chēng) |
MYB 2-3.76 |
| 商品貨號(hào) |
B165210 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against myb (v-myb and chicken c-myb) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against myb (v-myb and chicken c-myb) |
| Comments |
Mice were immunized with bp37v.myb protein. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody does not block the binding of MYB 2-7.77 (ATCC CRL-1724) or MYB 2-37.63 (ATCC CRL-1726). Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG2b |
| Name of Depositor |
J Bishop, GI Evan |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Evan GI, et al. Isolation of monoclonal antibodies specific for products of avian oncogene myb. Mol. Cell. Biol. 4: 2843-2850, 1984. PubMed: 6084811
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