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NCI-H2405 [H2405]
NCI-H2405 [H2405]
規(guī)格:
貨期:
編號(hào):B165335
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
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產(chǎn)品名稱 NCI-H2405 [H2405]
商品貨號(hào) B165335
Organism Homo sapiens, human
Tissue lung
Product Format frozen
Culture Properties mixed, adherent and suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease stage 4, adenocarcinoma; non-small cell lung cancer
Age 47 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Derivation
The line was established in September 1990 from ascites, a metastatic site.
Clinical Data
The patient was a smoker.
75 pack years.
Complete Growth Medium The base medium for this cell line is RPMI-1640 Medium (ATCC 30-2001).To make the complete growth medium, add the following components to the base medium: Fetal Bovine Serum (FBS; ATCC 30-2020) to a final concentration of 5%.
Subculturing Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium. Cultures grow as floating aggregates of round cells and as attached epithelial-like cells. Attached cells may be subcultured using 0.25% trypsin - 0.53mM EDTA.
Subcultivation Ratio: 1:5 to 1:8 is recommended.
Medium Renewal: Add fresh medium as cell density increases.
Cryopreservation
85% Culture Media + 10% FBS+ 5% DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Name of Depositor AF Gazdar, JD Minna
Deposited As Homo sapiens
Year of Origin 1990
References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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