| 產(chǎn)品名稱(chēng) |
NCI-H847 [H847] |
| 商品貨號(hào) |
B165374 |
| Organism |
Homo sapiens, human |
| Tissue |
lung; Derived from metastatic site: pleural effusion |
| Product Format |
frozen |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
stage L,carcinoma; classic small cell lung cancer |
| Age |
63 years |
| Gender |
male |
| Ethnicity |
Caucasian, White |
| Applications |
The line was established in October 1984. |
| Derivation |
The line was established in October 1984. |
| Clinical Data |
The patient received prior chemotherapy and radiation therapy. The patient was a smoker. male Caucasian, White 63 years |
| Comments |
The line was established in October 1984. The patient received prior chemotherapy and radiation therapy. The patient was a smoker. 40. |
| Complete Growth Medium |
HITES medium supplemented with 5% fetal bovine serum
The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium- 0.005 mg/ml Insulin
- 0.01 mg/ml Transferrin
- 30nM Sodium selenite (final conc.)
- 10 nM Hydrocortisone (final conc.)
- 10 nM beta-estradiol (final conc.)
- extra 2mM L-glutamine (for final conc. of 4.5 mM)
- 5% fetal bovine serum (final conc.)
|
| Subculturing |
Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density) Cultures can be maintained by the addition of fresh medium. Cells grow as floating aggregates of round cell clusters. |
| Cryopreservation |
Complete culture medium, 90%; additional fetal bovine serum, 5%; DMSO, 5%. |
| Culture Conditions |
Temperature: 37.0°C |
| Name of Depositor |
AF Gazdar, JD Minna |
| Deposited As |
Homo sapiens |
| Year of Origin |
1984 |
| References |
NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.
|
