| 產(chǎn)品名稱 |
P388D1 [P388 D1] |
| 商品貨號(hào) |
B165453 |
| Organism |
Mus musculus, mouse |
| Cell Type |
monocyte; macrophage |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
lymphoma |
| Strain |
DBA/2 |
| Applications |
transfection host |
| Storage Conditions |
liquid nitrogen vapor phase |
| Tumorigenic |
Yes |
| Effects |
Yes, in nude mice
Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells. |
| Comments |
A subclone of this line [see ATCC TIB-63, P388D1(IL-1)] produces high levels of interleukin-1 (IL-1, interleukin 1). Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
|
| Subculturing |
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 2 - 5 x 105 viable cells/mL.
A maximum of 6 x 106 viable cells/mL is obtainable.
Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C |
| Name of Depositor |
EM Jensen |
| Deposited As |
Mus musculus |
| References |
Dawe CJ, Potter M. Morphologic and biologic progression of a lymphoid neoplasm of the mouse in vivo and in vitro. Am. J. Pathol. 33: 603, 1957
Standard Practice for Testing for Biological Responses to Particles in Vitro. West Conshohocken, PA:ASTM International;ASTM Standard Test Method F 1903-98R03.
|
| Cross References |
Nucleotide (GenBank) :
U88624
Mus musculus 85 kDa calcium-independent phospholipase A2 mRNA, complete cds.
|
