| 產(chǎn)品名稱 | PC-3 |
|---|---|
| 商品貨號(hào) | B165488 |
| Organism | Homo sapiens, human |
| Tissue | prostate; derived from metastatic site: bone |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent (The cells form clusters in soft agar and can be adapted to suspension growth) |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | grade IV, adenocarcinoma |
| Age | 62 years adult |
| Gender | male |
| Ethnicity | Caucasian |
| Applications | This cell line is a suitable transfection host. |
| Storage Conditions | liquid nitrogen vapor phase |
| Karyotype | The line is near-triploid with a modal number of 62 chromosomes. There are nearly 20 marker chromosomes commonly found in each cell; and normal N2, N3, N4, N5, N12, and N15 are not found. No normal Y chromosomes could be detected by Q-band analysis. |
| Images |  |
| Derivation | The PC-3 was initiated from a bone metastasis of a grade IV prostatic adenocarcinoma from a 62-year-old male Caucasian. |
| Clinical Data | 62 years adult Caucasian male The PC-3 was initiated from a bone metastasis of a grade IV prostatic adenocarcinoma from a 62-year-old male Caucasian. |
| Antigen Expression | HLA A1, A9 |
| Genes Expressed | HLA A1, A9 |
| Tumorigenic | Yes |
| Effects | Yes, in semi-solid medium Yes, tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells. |
| Comments | The cells exhibit low acid phosphatase and testosterone-5-alpha reductase activities. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile | Amelogenin: X CSF1PO: 11 D13S317: 11 D16S539: 11 D5S818: 13 D7S820: 8,11 THO1: 6,7 TPOX: 8,9 vWA: 17 |
| Name of Depositor | ME Kaighn |
| Deposited As | Homo sapiens |
| References | Kaighn ME, et al. Establishment and characterization of a human prostatic carcinoma cell line (PC-3). Invest. Urol. 17: 16-23, 1979. PubMed: 447482 Chen TR. Chromosome identity of human prostate cancer cell lines, PC-3 and PPC-1. Cytogenet. Cell Genet. 62: 183-184, 1993. PubMed: 8428522 Ohnuki Y, et al. Chromosomal analysis of human prostatic adenocarcinoma cell lines. Cancer Res. 40: 524-534, 1980. PubMed: 7471073 Sheng S, et al. Maspin acts at the cell membrane to inhibit invasion and motility of mammary and prostatic cancer cells. Proc. Natl. Acad. Sci. USA 93: 11669-11674, 1996. PubMed: 8876194 Umekita Y, et al. Human prostate tumor growth in athymic mice: inhibition by androgens and stimulation by finasteride. Proc. Natl. Acad. Sci. USA 93: 11802-11807, 1996. PubMed: 8876218 Carter RE, et al. Prostate-specific membrane antigen is a hydrolase with substrate and pharmacologic characteristics of a neuropeptidase. Proc. Natl. Acad. Sci. USA 93: 749-753, 1996. PubMed: 8570628 Nupponen NN, et al. Genetic alterations in prostate cancer cell lines detected by comparative genomic hybridization. Cancer Genet. Cytogenet. 101: 53-57, 1998. PubMed: 9460501 Geiger T, et al. Antitumor activity of a PKC-alpha antisense oligonucleotide in combination with standard chemotherapeutic agents against various human tumors transplanted into nude mice. Anticancer Drug Des. 13: 35-45, 1998. PubMed: 9474241 Su ZZ, et al. Surface-epitope masking and expression cloning identifies the human prostate carcinoma tumor antigen gene PCTA-1 a member of the galectin gene family. Proc. Natl. Acad. Sci. USA 93: 7252-7257, 1996. PubMed: 8692978 The cells form clusters in soft agar and can be adapted to suspension growth The cells form clusters in soft agar and can be adapted to suspension growth |
| Cross References | Nucleotide (GenBank) : X94216 H.sapiens mRNA for VEGF-C protein. |
| 序號(hào) | 文章標(biāo)題 | 操作 |
|---|---|---|
| 1 | ATCC細(xì)胞運(yùn)輸和處理 | 查看詳情 |
| 2 | 其他海外品牌代理 | 查看詳情 |
| 3 | ATCC代理 | 查看詳情 |
| 4 | 購買須知 | 查看詳情 |
| 5 | 訂購流程 | 查看詳情 |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
