久久精品女人天堂?V麻,久久99精品久久久久久齐齐,国产精品天干天干在线观看,?级毛片久久免费观看,国产美女在线精品免费观看,日韩欧美啪啪一区二区毛片,久久精品99国产亚洲?v成人,最新熟妇精品国产偷自在线,国产老妇多毛精品一区二区

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > lambdaDL10
最近瀏覽歷史
聯系我們
  • 0574-87157013
  • [email protected]
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
lambdaDL10
lambdaDL10
規(guī)格:
貨期:
編號:B228019
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱 lambdaDL10
商品貨號 B228019
Designations lambdaDL10
Depositors B Windle
Biosafety Level 1
Vector Information
Size (kb): 39.0000000000000000
Vector: lambdaDL10 (phage, lambda - replacement)
Promoters: Promoter lac
Construction: pUC9, lambdaD69, M13mp10
Construct size (kb): 39.0
Features: insert detection: lacZ'
promoter: lac
replicon: lambda
terminator: none
enhancer: none
Applications
expression vector
vector for constructing cDNA libraries
vector permitting construction of fusion proteins
Comments
A BamHI digest removes a 930 bp fragment containing the lacZalpha coding frame. Thus use of BamHI as a cloning site and selection of clear plaques on Xgal media may not indicate the presence of a recombinant insert.
Escherichia coli JM109 is not a good host for this vector.
If HindIII, SacI, XbaI, XhoI, or BamHI are used as cloning sites, the vector can accept fragments of 0 - 12 kb. Inserts in HindIII, SacI, XbaI, BamHI, and SalI inactivate lacZ.
If SalI is used as a cloning site, the vector can accept fragments of 9 - 20 kb. Replacement of the SalI fragment with a DNA insert results in a lambdacIII- phage that forms virtually clear plaques.
A 435 bp HaeII fragment of pUC9, encoding beta-galactosidase, was inserted into the HindIII site of lambdaD69 to produce lambdaDL9. The multiple cloning sites of M13mp10 were crossed into lambdaDL9 to produce lambdaDL10.
lambdaDL10 (ATCC 37489) and lambdaDL11 (ATCC 37490) have opposite orientations of the restriction sites within lacZ.
References

Windle BE. Phage lambda and plasmid expression vectors with multiple cloning sites and lacZ alpha-complementation. Gene 45: 95-99, 1986. PubMed: 2946626

Brad Windle, personal communication

Shipped freeze-dried
梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479
乐清市| 陈巴尔虎旗| 永城市| 遂昌县| 毕节市| 姜堰市| 光山县| 泗洪县| 丹东市| 丽水市| 鹤峰县| 盖州市| 尉氏县| 平邑县| 双流县| 宝鸡市| 弋阳县| 云阳县| 玛曲县| 即墨市| 河池市| 美姑县| 平泉县| 哈密市| 娄底市| 鄢陵县| 锡林浩特市| 汉阴县| 平湖市| 攀枝花市| 云林县| 商洛市| 延长县| 凤山县| 德钦县| 洱源县| 泸水县| 营山县| 清流县| 祥云县| 怀安县|