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pUC931
pUC931
規(guī)格:
貨期:
編號:B228167
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pUC931
商品貨號 B228167
Designations pUC931
Depositors I Oberbaumer
Biosafety Level 1
Vector Information
Size (kb): 2.7100000381469730
Vector: pUC931 (plasmid)
Promoters: Promoter lac
Construction: pUC9, M13tg131
Marker(s):ampR
Construct size (kb): 2.710000038146973
Features: insert detection: lacZ'
marker(s): ampR
promoter: lac
replicon: pMB1
Applications
expression vector
vector containing primer sites useful for sequencing
vector for constructing cDNA libraries
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): PstI--2.7; HindIII--2.7; EcoRI--2.7; NheI--2.7.
Expression vector useful for construction of cDNA libraries and containing priming sites useful for sequencing. Permits visual detection of recombinants by beta-galactosidase alpha peptide complementation.
pUC830 (ATCC 77218) and pUC931 (ATCC 77219) differ in the orientation of the polylinker.
During construction, the original HindIII site of the pUC9 vector gave rise to a TAG stop codon which could be suppressed in strains which are supF and/or supE.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Oberbaumer I. New pUC-derived expression vectors for rapid construction of cDNA libraries. Gene 49: 81-91, 1986. PubMed: 2436975

Oberbaumer I. Blue libraries are beautiful. Focus 14: 72, 1992.

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