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ET1c [lambda-EZZ-gamma3]
ET1c [lambda-EZZ-gamma3]
規(guī)格:
貨期:
編號:B228339
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱 ET1c [lambda-EZZ-gamma3]
商品貨號 B228339
Designations ET1c [lambda-EZZ-gamma3]
Species Homo sapiens, human
Applications
For Northern blots, the 1.2 or 0.9 kb EcoRI fragment is recommended.
Vector
Construct size (kb): 5.699999809265137
Insert
DNA: cDNA
Insert lengths(kb): 3.0
Tissue: umbilical vein endothelium, LPS-stimulated
Gene product: endothelin 1 [EDN1]
Alleles: A1, B1, B2, A2, B3, B4
Insert Size (kb) 3.0
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 1.2, 0.9, 0.9; HindIII--4.0, 1.7; SspI--2.3, 2.3, 0.7, 0.3; AvaI--4.3, 1.4; BglI--4.5, 1.2.
The insert contains the following restriction sites (approximate kb from the 5' end): HindIII--0.75; EcoRI--1.2; SspI--1.79, 2.04.
Because of the internal EcoRI site, the insert cannot be excised intact. For Northern blots, the 1.2 or 0.9 kb EcoRI fragment is recommended.
This insert contains two identical copies of 3' 0.9 kb EcoRI fragments.
Enzyme(s) not detecting polymorphism: AvaII, BamHI, BanII, BclI, BglII, EcoRI, HindIII, MspI, NruI, PstI, SstI, StyII, XbaI, and XhoI (16 unrelated individuals).
References

Bloch KD, et al. Structural organization and chromosomal assignment of the gene encoding endothelin. J. Biol. Chem. 264: 10851-10857, 1989. PubMed: 2659594

Hoehe MR, et al. TaqI identifies a four allele DNA polymorphism of the human endothelin-1 gene (EDN1). Nucleic Acids Res. 19: 3161, 1991. PubMed: 2057379

Kenneth D Bloch, personal communication

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