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pSP65:TPMV #6 [full-length clone of tomato planta macho viroid]
pSP65:TPMV #6 [full-length clone of tomato planta macho viroid]
規(guī)格:
貨期:
編號:B228534
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pSP65:TPMV #6 [full-length clone of tomato planta macho viroid]
商品貨號 B228534
Designations pSP65:TPMV #6 [full-length clone of tomato planta macho viroid]
Species Tomato planta macho viroid
Depositors RA Owens, Beltsville Agricultural Research Center
Applications
Noninfectious unless TPMV cDNA insert is released by BamHI. To use as a template, plasmid must be linearized with HindIII.
Vector Name of construct: pSP65:TPMV #6
Excise insert: BamHI
Size of construct (kb): 3.36
Markers: ampR
Name of vector: pSP65
Intact vector size: 3.010
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI EcoRI
Polylinker sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI EcoRI
Host range: Escherichia coli

Features (with orientation and position when available):
enhancer: none
marker(s): ampR
promoter: SP6
replicon: pMB1
terminator: none
Insert Genome: tomato planta macho viroid
Gene name: genome, full-length
Contains complete coding sequence?: U
Type of DNA: cDNA
Insert end: BamHI
Insert end: BamHI
Insert size (kb): 0.36
Insert Size (kb) 0.36
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
The insert contains an internal SalI site. EcoRI + SalI or HindIII + SalI are diagnostic for the insert orientation.
Restriction digests of the clone give the following sizes (kb): BamHI--3.1, 0.36; HindIII--3.4; EcoRI--3.4.
Noninfectious unless TPMV cDNA insert is released by BamHI. To use as a template, plasmid must be linearized with HindIII.
Full-length (360 bp) TPMV cDNA with BamHI ends was cloned into BamHI of pSP65. Constructed by R.W. Hammond.
Classification Pospiviroid
References

Kiefer MC, et al. Structural similarities between viroids and transposable genetic elements. Proc. Natl. Acad. Sci. USA 80: 6234-6238, 1983. PubMed: 6312450

Robert A Owens, personal communication

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
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于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
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