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pBlueBac/PKCdelta
pBlueBac/PKCdelta
規(guī)格:
貨期:
編號:B228558
品牌:Mingzhoubio

標(biāo)準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pBlueBac/PKCdelta
商品貨號 B228558
Designations pBlueBac/PKCdelta
GenBank Number

L07860

Species Homo sapiens, human
Applications
in another host, produces protein protein kinase C, delta
Vector
Construct size (kb): 16.10000038146973
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBlueBac
Intact vector size: 14.000
Type of vector: phagemid
Vector end: NheI
Vector end: NheI
Cloning sites: NheI
Polylinker sites:
Host range: insect cells; Escherichia coli
Features (with orientation and position when available):
insert detection: lac
marker(s): ampR
promoter: polyhedrin
replicon: pMB1, f1, SV40
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: human
Gene symbol: PRKCD
Gene name: protein kinase C, delta
Contains complete coding sequence?: Y
Localization: 3
Tissue: liver, HepG2 cell line
Type of DNA: cDNA
Insert end: Modification: NheI linkers
Insert end: Modification: NheI linkers
Insert size (kb): 2.1
Cross references: DNA Seq. Acc.: L07860
Insert lengths(kb): 2.099999904632568
Tissue: liver, HepG2 cell line
Gene product: protein kinase C, delta [PRKCD]
Target Gene: protein kinase C, delta
Insert Size (kb) 2.100
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): NheI--14.0, 2.1; BglI--5.6, 5.4, 2.2, 1.8, 1.2; HindIII--8.8, 4.0, 1.3, 1.1, 1.0; NcoI--14.0, 1.25, 0.5, 0.35; PstI--14.0, 1.1, 0.55, 0.3.
Insert contains the following restriction sites (approximate kb from the 5' end): BglI--1.13; BglII--1.01; HindIII--0.85; NcoI--1.18, 1.64, 1.96; PstI--0.05, 0.33, 1.39; SalI--0.17.
Contains the complete coding sequence, and begins at the initiation codon (nt 59 of the sequence record).
References

Aris JP, et al. Molecular and biochemical characterization of a recombinant human PKC-delta family member. Biochim. Biophys. Acta 1174: 171-181, 1993. PubMed: 8357834

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胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
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沈經(jīng)理 19548299266 2662369050
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