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pXPGK-RI0.9
pXPGK-RI0.9
規(guī)格:
貨期:
編號:B228855
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pXPGK-RI0.9
商品貨號 B228855
Designations pXPGK-RI0.9
Species Homo sapiens, human
Applications
The smaller EcoRI fragment has been used as the probe.
Vector
Construct size (kb): 5.260000228881836
Insert
DNA: genomic
Insert lengths(kb): 0.8999999761581421
Gene product: phosphoglycerate kinase 1 [PGK1]
Alleles: A1, A1, A2, A2
Insert Size (kb) 0.900
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.2, 0.74; PvuII--4.9; EcoRI/PvuII--2.4, 1.5, 0.79; BamHI--uncut; HindIII--uncut; AccI--3.3, 1.7.
This PstI RFLP is in strong linkage disequilibrium (87% of maximal) with the 5' BglI RFLP detected at this locus by pSPT-PGK.
Insert contains the last 83 bp of exon 4 and extends 820 bp into the fourth intron of human PGK1.
Occasionally a faint 5.2 kb autosomal band is detected with the PstI RFLP.
The smaller EcoRI fragment has been used as the probe.
Based on the restriction data and the tetracycline sensitivity of this construct, the vector must not be the reported pBR322.
References

Smead DL, et al. RFLP's in human X-linked PGK1: A new probe for the PstI RFLP demonstrates strong linkage disequilibrium with the BglI RFLP. Nucleic Acids Res. 17: 7551, 1989. PubMed: 2571982

Merry DE, et al. Choroideremia and deafness with stapes fixation: a contiguous gene deletion syndrome in Xq21. Am. J. Hum. Genet. 45: 530-540, 1989. PubMed: 2491012

Jennifer M Puck, personal communication

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