久久精品女人天堂?V麻,久久99精品久久久久久齐齐,国产精品天干天干在线观看,?级毛片久久免费观看,国产美女在线精品免费观看,日韩欧美啪啪一区二区毛片,久久精品99国产亚洲?v成人,最新熟妇精品国产偷自在线,国产老妇多毛精品一区二区

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > Flabellula citata Schaeffer
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • [email protected]
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
Flabellula citata Schaeffer
Flabellula citata Schaeffer
規(guī)格:
貨期:
編號:B228889
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Flabellula citata Schaeffer
商品貨號 B228889
Strain Designations BSJ-03190019
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
sediment from man-made marsh island at edge of dredge spoilage site, Beaufort, NC
Product Format frozen
Type Strain no
Medium ATCC® Medium 1525: Seawater 802 medium
Growth Conditions
Temperature: 25.0°C
Duration: grown with Klebsiella pneumoniae subsp. pneumoniae ATCC 700831 and mixed bacteria
Cryopreservation
Cryoprotective Solution

DMSO ?????????????????????????????????????????????????????????????????????????????????? 2.0 ml

Fresh growth medium w/o bacteria???????????????????????????????? 8.0 ml

1.?? Mix the components in the order listed. When the medium is added to the DMSO the solution will warm up due to chemical heat.

2. ? Harvest cells from a culture that is at or near peak density by filtration and centrifugation at 800 x g for 5 min.

3. Adjust the concentration of cells at least 2 x 106/ml in fresh medium.

4.? Mix the cell preparation and the cryoprotective solution in equal portions.

5.? Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation).

6. Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1 C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.? Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.? (The cooling rate in this apparatus is approximately

-1°C/min.) ?

7.? Ampules are stored in either the vapor or liquid phase of a nitrogen refrigerator.

8. To establish a culture from the frozen state place the vial in a 35°C water bath.? Immerse the vial to a level just above the surface of the frozen material. Do not agitate the vial.

Immediately after thawing, do not leave in water bath,???????? aseptically remove the contents of the ampule and inoculate into a T-25 tissue culture flask containing 10 ml ATCC medium 1525 bacterized with Klebsiella pneumoniae subsp. pneumoniae (ATCC? ?700831).

9.   Incubate at 25°C with the cap screwed on tightly.

10. Once the culture is established, vigorously agitate the flask and aseptically transfer 0.5 ml to 10.0 ml of bacterized ATCC medium 1525.

11. Follow the protocol for maintenance of culture.

Name of Depositor TA Nerad
References

Thomas A Nerad, personal communication

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
大荔县| 泾阳县| 贞丰县| 内江市| 平安县| 玛沁县| 洛浦县| 德惠市| 通城县| 册亨县| 高州市| 宁河县| 辽中县| 巩义市| 赤水市| 射洪县| 禄劝| 瑞昌市| 宁阳县| 永年县| 宝应县| 新河县| 信阳市| 祁门县| 陵水| 阿克苏市| 重庆市| 吕梁市| 漠河县| 平舆县| 遵化市| 平顶山市| 穆棱市| 彭泽县| 武乡县| 三原县| 鹿邑县| 隆化县| 武鸣县| 治多县| 温州市|