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p680
p680
規(guī)格:
貨期:
編號(hào):B228944
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 p680
商品貨號(hào) B228944
Designations p680
Depositors F Spencer
Biosafety Level 1
Vector Information
Size (kb): 6.9000000953674320
Vector: p680 (plasmid)
Promoters: Promoter for in vitro transcription T7
Marker(s):ampR
Construct size (kb): 6.900000095367432
Features: insert detection: lacZ
marker(s): ampR
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: f1(+)
replicon: ori
coding sequence: CYH2
coding sequence: LEU2
Applications
cloning vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--3.2, 2.2, 1.4; EcoRI--3.1, 2.0, 1.8; KpnI--5.2, 1.8; PvuI--5.0, 2.0.
Vector contains the following restriction sites: EcoRV--1.01,3.20,5.64; BstXI--1.04,3.15; EcoRI--1.12,5.94; KpnI--1.51,3.23; ClaI--1.60,3.30; NacI--2.79; PvuI--2.97,4.88; PvuII--3.00,3.45; ScaI--3.12,4.99; AatII--5.44.
Two-step replacement in budding yeast. LEU2 gene provides positive selection for the integrative step; CYH2S provides negative selection for mitotic recombinants that have excised the plasmid sequences. Can be used for modification of YACs.
The fragments were ligated in the plasmid: LEU2 -> f1(+) -> lacZ -> T7/T3 -> ori -> ampR -> CYH2 ...
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Spencer F, et al. Targeted recombination-based cloning and manipulation of large DNA segments in yeast. Methods 5: 161-175, 1993.

Shipped freeze-dried
梅經(jīng)理 17280875617 1438578920
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