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Gb5-CHE
Gb5-CHE
規(guī)格:
貨期:
編號:B229354
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Gb5-CHE
商品貨號 B229354
Designations Gb5-CHE
Species Homo sapiens, human
Depositors H Soreq
Applications
The full-length insert has been used to detect a single 2.4-2.6 kb transcript in human brain and liver.
Vector
Construct size (kb): 0.0
Insert
DNA: cDNA
Insert lengths(kb): 2.700000047683716
Tissue: neuroblastoma and glioblastoma
Gene product: butyrylcholinesterase(cholinesterase (serum) 1) [BCHE]
Insert Size (kb) 2.700
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried bacteria-free lysate
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--25.0, 13.0, 2.1, 0.7; HindIII--22.0, 9.0, 5.6, 3.8, 2.45; BamHI--18.0, 9.6, 6.0, 5.6, 2.5, 1.4;
PstI--12.0, 8.6, 5.6, 4.4, 2.5, 2.3, 1.8, 1.6, 1.2, 0.9, 0.75, 0.5; AvaI--13.0, 9.0, 4.2 (doublet), 3.7 (doublet), 1.8, 1.0.
The insert contains the following restriction sites (in approximate kb from the 5' end): BamHI--0.6; BalI--1150; HincII--1375; EcoRI--2150, HindIII--2400.
The 3' 0.72 kb EcoRI fragment localizes specifically to 3q26-qter by in situ hybridization. The full length cDNA detects CHE1, CHEL2 and CHEL3 on chromosome 16, but with lower efficiency than chromosome 3.
The full-length insert has been used to detect a single 2.4-2.6 kb transcript in human brain and liver. It detects an additional 4.0 kb transcript in human glioblastomas and neuroblastomas (about 10% intensity).
References

Gnatt A, et al. Expression of alternatively terminated unusual human butyrylcholinesterase messenger RNA transcripts, mapping to chromosome 3q26-ter, in nervous system tumors. Cancer Res. 50: 1983-1987, 1990. PubMed: 2317787

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