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pTC4.5
pTC4.5
規(guī)格:
貨期:
編號:B229496
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pTC4.5
商品貨號 B229496
Designations pTC4.5
GenBank Number

L22484

Species Trypanosoma cruzi Chagas
Depositors LM Ruiz-Perez
Vector
Construct size (kb): 7.190000057220459
Insert
DNA: genomic
Insert lengths(kb): 4.5
Gene product: dihydrofolate reductase-thymidylate synthase [DHFR-TS]
Insert Size (kb) 4.5
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/PstI--4.6, 2.8; HindIII--7.4; SalI--7.4.
The insert contains the following restriction sites (approximate from the 5' end): BstXI--3.95; NcoI--2.52; SalI--2.48; SphI--2.42, 2.76, 3.65; XhoI--2.79.
The insert contains the following components (listed 5'->3'): a PstI/SalI fragment from the MCS of lambda EMBL3, 2.4 kb upstream of the coding sequence for DHFR-TS, the 1.56 kb coding region, and 0.44 kb downstream of DHFR-TS.
All but 100 bp of the coding sequence is in a 1.9 kb SalI/EcoRI fragment.
Classification KINGDOM: Protozoa
References

Reche P, et al. Cloning and expression of the dihydrofolate reductase-thymidylate synthase gene from Trypanosoma cruzi. Mol. Biochem. Parasitol. 65: 247-258, 1994. PubMed: 7969266

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